The Anti-Histone H1.0 Antibody (CAB3298) is a crucial tool for researchers studying the role of histones in gene regulation and chromatin structure. This polyclonal antibody, validated for knockout specificity, is specifically designed to target the Histone H1.0 protein and is highly reactive with human samples.Histone H1.0 is a variant of the histone H1 family that is involved in the packaging of DNA into nucleosomes and plays a critical role in the regulation of gene expression. By targeting Histone H1.0, researchers can investigate its role in chromatin organization and epigenetic regulation.
This antibody is ideal for use in Western blot applications, allowing for the detection and analysis of Histone H1.0 in various cell types and tissues. Its specificity and sensitivity make it a valuable tool for studies in epigenetics, gene regulation, and cancer research.By understanding the function of Histone H1.0 and its impact on chromatin structure, researchers can gain insights into the mechanisms underlying gene expression and potentially develop targeted therapies for diseases related to epigenetic dysregulation.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.
Purification Method:
Affinity purification
Gene ID:
3005
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Histone H1.0 knockout (KO) 293T cells, using [KO Validated] Histone H1.0 Rabbit pAb (CAB3298) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
