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[KO Validated] Histone H1.0 Rabbit Polyclonal Antibody (CAB3298)

SKU:
CAB3298
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-Histone H1.0 Antibody CAB3298KO Validated
  • KO Validated Antibodies 1 Anti-Histone H1.0 Antibody CAB3298KO Validated
  • KO Validated Antibodies 1 Anti-Histone H1.0 Antibody CAB3298KO Validated
  • KO Validated Antibodies 1 Anti-Histone H1.0 Antibody CAB3298KO Validated
  • KO Validated Antibodies 1 Anti-Histone H1.0 Antibody CAB3298KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Histone H1.0 Rabbit Polyclonal Antibody
SKU:CAB3298
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human H1F0 (NP_005309.1).
Sequence:MTEN STSA PAAK PKRA KASK KSTD HPKY SDMI VAAI QAEK NRAG SSRQ SIQK YIKS HYKV GENA DSQI KLSI KRLV TTGV LKQT KGVG ASGS FRLA KSDE PKKS V
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:H10; H1.0; H1F0; H1FV; .0
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:21kDa
Observed MW:30kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Purification Method:Affinity purification
Gene ID:3005
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Histone H1.0 knockout (KO) 293T cells, using [KO Validated] Histone H1.0 Rabbit pAb (CAB3298) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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