The Anti-HIST1H1A Antibody (CAB20522) is a valuable tool for researchers studying HIST1H1A, a histone protein involved in chromatin structure and gene regulation. This polyclonal antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. HIST1H1A, also known as histone H1.1, plays a crucial role in DNA packaging and gene expression regulation. Its involvement in chromatin remodeling makes it an important target for investigations into cancer, epigenetics, and gene regulation.
By specifically binding to the HIST1H1A protein, this antibody enables precise detection and analysis in various cell types, making it an excellent tool for research in molecular biology and cancer biology.Understanding the function and regulation of HIST1H1A is essential for unraveling its impact on cellular processes and disease development. By studying the role of HIST1H1A, researchers can gain valuable insights into mechanisms of gene expression control and potential therapeutic targets for diseases such as cancer and genetic disorders.
Product Name:
HIST1H1A Rabbit Polyclonal Antibody
SKU:
CAB20522
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 116-168 of human HIST1H1A (NP_005316.1).
Sequence:
SVET KPGA SKVA TKTK ATGA SKKL KKAT GASK KSVK TPKK AKKP AATR KSSK N
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
H1A; H1.1; H1F1; HIST1; HIST1H1A
Positive Sample:
HepG2,Jurkat,Mouse spleen,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
cell surface, nucleoplasm, nucleus
Calculated MW:
22kDa
Observed MW:
22kDa
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
3024
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HIST1H1A antibody (CAB20522) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
