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HIST1H1A Rabbit Polyclonal Antibody (CAB20522)

SKU:
CAB20522
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
H1A
H1.1
H1F1
HIST1
HIST1H1A
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:HIST1H1A Rabbit Polyclonal Antibody
SKU:CAB20522
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 116-168 of human HIST1H1A (NP_005316.1).
Sequence:SVET KPGA SKVA TKTK ATGA SKKL KKAT GASK KSVK TPKK AKKP AATR KSSK N
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:H1A; H1.1; H1F1; HIST1; HIST1H1A
Positive Sample:HepG2,Jurkat,Mouse spleen,Mouse thymus
Conjugate:Unconjugated
Cellular Localization:cell surface, nucleoplasm, nucleus
Calculated MW:22kDa
Observed MW:22kDa

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Purification Method:Affinity purification
Gene ID:3024
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using HIST1H1A antibody (CAB20522) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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