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Hd1 Rabbit Polyclonal Antibody (CAB20268)

SKU:
CAB20268
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
Hd1
SE1
OsHd1
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Hd1 Rabbit Polyclonal Antibody
SKU:CAB20268
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Oryza sativa
Immunogen:Recombinant protein of Oryza sativa Hd1 .
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:Hd1; SE1; OsHd1; Hd1
Positive Sample:stems (before flower),leaves sheath (before flower),flag leaves (after flower)
Conjugate:Unconjugated
Cellular Localization:nucleus
Calculated MW:43kDa
Observed MW:50kDa

Probable transcription factor involved in the regulation of flower development. Required for the promotion of flowering under short day (SD) conditions and the suppression of flowering under long day (LD) conditions. Regulates positively the floral activator HEADING DATE 3a (HD3A) under SD and negatively under LD conditions. The GIGANTEA-CONSTANS-FLOWER LOCUS T (GI-CO-FT) pathway to control photoperiodic flowering under LD is conserved between Arabidopsis and rice, but the regulation of the downstream gene by the upstream regulatory gene is reversed in the two species. In Arabidopsis, GI acts as an activator of CO, which in turn activates the floral activator FT under LD conditions. In rice, GI activates HD1/CO in a similar manner to that in Arabidopsis. However, under LD conditions, HD1 suppresses HD3A/FT expression, causing the suppression of flowering.

Purification Method:Affinity purification
Gene ID:4340746
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Hd1 Rabbit pAb (CAB20268) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.

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