The Anti-HCoV-NL63 Spike S2 Antibody (CAB20611) is a valuable tool for researchers studying the HCoV-NL63 virus, a human coronavirus known to cause respiratory infections. This antibody, raised against the Spike S2 protein of the virus, is highly specific and reactive with human samples. It is validated for use in various applications, including immunofluorescence and ELISA.The Spike S2 protein is an important component of the HCoV-NL63 virus, involved in viral entry and infectivity. By targeting this protein with the CAB20611 antibody, researchers can detect and study the virus in infected cells, tissues, and patient samples.
This antibody is essential for understanding the molecular mechanisms of HCoV-NL63 infection and developing strategies for diagnosis and treatment.Overall, the CAB20611 Anti-HCoV-NL63 Spike S2 Antibody is a valuable tool for researchers working on coronaviruses and respiratory infections. Its specificity, sensitivity, and versatility make it ideal for a wide range of research applications in virology, immunology, and infectious disease.
Product Name:
HCoV-NL63 Spike S2 Rabbit Polyclonal Antibody
SKU:
CAB20611
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
HCoV-NL63
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of coronavirus Spike S2 (YP_003767.1).
Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag)
Conjugate:
Unconjugated
Calculated MW:
150kDa
Observed MW:
160kDa
S1 region attaches the virion to the cell membrane by interacting with host ACE2, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method:
Affinity purification
Gene ID:
2943499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag), using HCoV-NL63 Spike S2 Rabbit pAb (CAB20611) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
