The Anti-HCoV-229E Spike S2 Antibody (CAB20394) is a vital tool for researchers studying Human Coronavirus 229E (HCoV-229E), a common respiratory virus that can cause mild to severe respiratory illness. This antibody, produced in rabbits, specifically targets the Spike S2 protein of the virus, allowing for accurate detection and analysis in various research applications.The Spike S2 protein is an important component of the HCoV-229E virus, playing a key role in viral entry into host cells. By targeting this protein, researchers can better understand the mechanisms of viral infection and potentially develop targeted therapies or vaccines.
The Anti-HCoV-229E Spike S2 Antibody is validated for use in Western blot applications, making it a valuable tool for studies in virology, immunology, and infectious disease research.Overall, the Anti-HCoV-229E Spike S2 Antibody (CAB20394) is a reliable and specific reagent for investigating the HCoV-229E virus and its Spike S2 protein. Its high reactivity with human samples and suitability for Western blot analysis make it an essential resource for researchers working to combat respiratory viruses and understand the underlying mechanisms of viral infection.
Product Name:
HCoV-229E Spike S2 Rabbit Polyclonal Antibody
SKU:
CAB20394
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
HCoV-229E
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1074-1173 of coronavirus Spike S2 (NP_073551.1).
Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag)
Conjugate:
Unconjugated
Calculated MW:
129kDa
Observed MW:
160kDa
S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method:
Affinity purification
Gene ID:
918758
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag), using HCoV-229E Spike S2 Rabbit pAb (CAB20394) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
