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HCoV-229E Spike S2 Rabbit Polyclonal Antibody (CAB20394)

SKU:
CAB20394
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:HCoV-229E Spike S2 Rabbit Polyclonal Antibody
SKU:CAB20394
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:HCoV-229E
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1074-1173 of coronavirus Spike S2 (NP_073551.1).
Sequence:LENK SAEL NYTV QKLQ TLID NINS TLVD LKWL NRVE TYIK WPWW VWLC ISVV LIFV VSML LLCC CSTG CCGF FSCF ASSI RGCC ESTK LPYY DVEK IHIQ
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Positive Sample:Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag)
Conjugate:Unconjugated
Calculated MW:129kDa
Observed MW:160kDa

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Purification Method:Affinity purification
Gene ID:918758
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag), using HCoV-229E Spike S2 Rabbit pAb (CAB20394) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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