The Anti-HCoV-229E Spike S1 Antibody (CAB20393) is a crucial tool for researchers studying human coronavirus 229E (HCoV-229E), a common cause of mild respiratory illnesses. This antibody, generated in rabbits, specifically targets the Spike S1 protein of the virus, which plays a key role in viral attachment and entry into host cells.Validated for use in various applications such as ELISA, immunofluorescence, and Western blot, this antibody allows for the detection and analysis of the HCoV-229E Spike S1 protein in human samples. Its high reactivity and specificity make it an invaluable resource for studying the molecular mechanisms of HCoV-229E infection, as well as for developing diagnostic tools and potential therapeutic interventions.
By targeting the Spike S1 protein, researchers can gain insights into the biology of HCoV-229E and its interactions with host cells, contributing to our understanding of how this virus causes disease and spreading. This antibody is a valuable asset for those working in virology, immunology, and infectious diseases, enabling them to advance knowledge and potentially develop strategies to combat HCoV-229E and other related coronaviruses.
Product Name:
HCoV-229E Spike S1 Rabbit Polyclonal Antibody
SKU:
CAB20393
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
HCoV-229E
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of coronavirus Spike S1 (NP_073551.1).
Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag)
Conjugate:
Unconjugated
Calculated MW:
129kDa
Observed MW:
160kDa
S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method:
Affinity purification
Gene ID:
918758
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag), using HCoV-229E Spike S1 Rabbit pAb (CAB20393) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
