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H3Q5ser Rabbit Polyclonal Antibody (CAB20210)

SKU:
CAB20210
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
H3/A
H3C2
H3C3
H3C4
H3C6
H3C7
H3C8
H3FA
H3C10
H3C11
H3C12
HIST1H3A
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:H3Q5ser Rabbit Polyclonal Antibody
SKU:CAB20210
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic serotonylated peptide around Q5 of human histone H3 (NP_003520.1).
Sequence:TKQT A
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:200
Synonyms:H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; H3Q5ser
Positive Sample:A-431,NIH/3T3
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:Refer to figures

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification Method:Affinity purification
Gene ID:8350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using H3Q5ser Rabbit pAb (CAB20210) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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