The Anti-GluR2/GluR3 Antibody (CAB2754) is a valuable tool for researchers studying the GluR2 and GluR3 subunits of the AMPA receptor, key players in excitatory synaptic transmission in the brain. This antibody, raised in rabbits, is highly specific and reacts with both human and mouse samples, making it versatile for a variety of experimental applications.The AMPA receptor is vital for synaptic plasticity, learning, and memory, and dysregulation of GluR2 and GluR3 subunits has been implicated in neurodegenerative disorders like Alzheimer's disease and epilepsy. This antibody binds specifically to GluR2 and GluR3 proteins, allowing for their detection and analysis in various cell types and tissues.
Its reliability in Western blot applications makes it an essential tool for researchers investigating synaptic transmission and neurological diseases.By targeting GluR2 and GluR3, researchers can gain insights into the mechanisms underlying synaptic plasticity and potentially develop new therapeutic strategies for neurological disorders. The Anti-GluR2/GluR3 Antibody provides a reliable means to study these important AMPA receptor subunits and their role in brain function and disease.
Product Name:
GluR2+GluR3 Rabbit Monoclonal Antibody
SKU:
CAB2754
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 784-883 of human GluR2+GluR3 (P42262).
asymmetric synapse, dendrite, dendritic spine, excitatory synapse, external side of plasma membrane, plasma membrane, postsynapse, postsynaptic density, postsynaptic density membrane, postsynaptic endocytic zone
Calculated MW:
98kDa
Observed MW:
100kDa
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene.Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties
Purification Method:
Affinity purification
Gene ID:
2891/2892
Clone Number:
ARC2668
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using GluR2+GluR3 Rabbit mAb (CAB2754) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
