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Gli3 Rabbit Monoclonal Antibody (CAB3300)

SKU:
CAB3300
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ACLS
GCPS
GLI3-190
GLI3FL
PAP-A
PAPA
PAPA1
PAPB
PHS
PPDIV
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Gli3 Rabbit Monoclonal Antibody
SKU:CAB3300
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3 (P10071).
Sequence:PNES AGSM VNGM QNQD PVGQ GYLA HQLL GDSM QHPG AGRP GQQM LGQI SATS HINI YQGP ESCL PGAH GMGS QPSS LAVV RGYQ PCAS FGGS RRQA MPRD SLAL QSGQ LSDT SQTC RVNG IKME MKGQ PHPL CSNL QNYS GQFY DQTV GFSQ QDTK AGSF SISD ASCL LQGT SAKN SELL SPGA NQVT STVD SLDS HDLE GVQI DFDA IIDD GDHS SLMS GALS PSII QNLS HSSS RLTT PRAS LPFP ALSM STTN MAIG DMSS LLTS LAEE SKFL AVMQ
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190; Gli3
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Axoneme, Ciliary base, Ciliary tip, Cilium, Cytoplasm, Cytosol, GLI-SUFU complex, Nuclear speck, Nucleolus, Nucleoplasm, Nucleus
Calculated MW:170kDa
Observed MW:190kDa

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.

Purification Method:Affinity purification
Gene ID:2737
Clone Number:ARC1941
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa cells, using Gli3 antibody (CAB3300) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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