The Gli3 Polyclonal Antibody (CAB3300) is a valuable tool for researchers studying the Gli3 protein, a key regulator of the Hedgehog signaling pathway involved in embryonic development and cell differentiation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Gli3 is a transcription factor that plays a critical role in controlling gene expression and cell fate determination. Dysregulation of Gli3 has been linked to a variety of developmental disorders and cancers, making it an important target for investigation in the fields of developmental biology and oncology.
The Gli3 Polyclonal Antibody enables researchers to detect and analyze Gli3 protein levels in different cell types, providing valuable insights into its function and regulation.By studying the role of Gli3 in various cellular processes and disease states, researchers can gain a better understanding of its significance and potential therapeutic implications. The Gli3 Polyclonal Antibody offers a reliable tool for investigating the complex functions of Gli3 and its impact on health and disease.
Product Name:
Gli3 Rabbit Monoclonal Antibody
SKU:
CAB3300
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1301-1580 of human Gli3 (P10071).
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
Purification Method:
Affinity purification
Gene ID:
2737
Clone Number:
ARC1941
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Gli3 antibody (CAB3300) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
