The GALNT4 Polyclonal Antibody (CAB4243) is a valuable tool for researchers studying GALNT4, an enzyme involved in protein glycosylation and cell signaling processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the GALNT4 protein, researchers can detect and analyze its expression in various cell types, making it ideal for investigations in cell biology and signal transduction pathways.GALNT4, also known as N-acetylgalactosaminyltransferase 4, plays a crucial role in post-translational modifications of proteins, impacting their function and localization within cells. Its involvement in glycosylation pathways makes it a key player in cell surface receptor signaling and cellular adhesion processes.
Understanding the function of GALNT4 is essential for unraveling its contributions to various physiological and pathological conditions, including cancer progression, immune response modulation, and developmental processes.Researchers studying GALNT4 can use this antibody to explore its role in cancer biology, cell adhesion mechanisms, and signal transduction pathways, providing valuable insights into its potential as a therapeutic target for disease intervention. By unraveling the molecular functions of GALNT4, researchers can deepen their understanding of its impact on cellular processes and its potential implications for human health.
Product Name:
GALNT4 Rabbit Polyclonal Antibody
SKU:
CAB4243
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-133 of human GALNT4 (NP_003765.2).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
67kDa
Observed MW:
67kDa
This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. Fusion transcripts, which combine part of this gene with the 5' exons of the neighboring POC1B (POC1 centriolar protein homolog B) gene, also exist.
Purification Method:
Affinity purification
Gene ID:
8693
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using GALNT4 Rabbit pAb (CAB4243) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
