The Anti-GAD67/GAD1 Antibody (CAB1475) is a valuable tool for researchers studying glutamate decarboxylase 67 (GAD67) and GAD1 proteins. These proteins are essential enzymes involved in the synthesis of the neurotransmitter gamma-aminobutyric acid (GABA), which plays a crucial role in the central nervous system.Raised in rabbits, this polyclonal antibody is highly specific and reactive with human samples, making it ideal for use in various experimental applications, including Western blotting. By binding to GAD67 and GAD1 proteins, this antibody allows for the detection and analysis of these key enzymes in different cell types.The GAD67/GAD1 Antibody is particularly valuable in neuroscience research, as abnormalities in GABA synthesis have been linked to neurological disorders such as epilepsy, schizophrenia, and Parkinson's disease.
By studying the expression and activity of GAD67 and GAD1 proteins, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets. Overall, the Anti-GAD67/GAD1 Antibody is a reliable tool for investigating the role of GAD enzymes in neurological function and dysfunction, paving the way for advancements in our understanding and treatment of neurological disorders.
Product Name:
GAD67/GAD1 Rabbit Monoclonal Antibody
SKU:
CAB1475
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GAD67/GAD1 (Q99259).
Axon terminus, Cell cortex, Cytoplasm, GABA-ergic synapse, Inhibitory synapse, Plasma membrane, Presynaptic active zone
Calculated MW:
67kDa
Observed MW:
70kDa
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Purification Method:
Affinity purification
Gene ID:
2571
Clone Number:
ARC1879
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GAD67/GAD1 Rabbit mAb (CAB1475) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
