The FMO3 Polyclonal Antibody (CAB19222) is a valuable tool for researchers studying FMO3, an enzyme responsible for the metabolism of xenobiotics and drugs in the liver. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the FMO3 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for research in pharmacology and toxicology.FMO3, a flavin-containing monooxygenase, plays a crucial role in the detoxification and elimination of foreign substances from the body.
Mutations in the FMO3 gene can lead to trimethylaminuria, also known as fish odor syndrome, a rare metabolic disorder that affects the body's ability to break down certain compounds. Studying the function and regulation of FMO3 with this antibody can provide insights into drug metabolism, drug interactions, and potential therapeutic strategies for metabolic disorders.
Product Name:
FMO3 Rabbit Monoclonal Antibody
SKU:
CAB19222
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human FMO3 (P31513).
Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.
Purification Method:
Affinity purification
Gene ID:
2328
Clone Number:
ARC2377
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using FMO3 Rabbit mAb (CAB19222) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
