The Anti-Fluorescein Isothiocyanate (FITC) Antibody (CAB20385) is a valuable tool for researchers studying fluorescence-labeled molecules in various applications. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting FITC-conjugated proteins. It has been validated for use in techniques such as immunofluorescence and flow cytometry, enabling precise visualization and analysis of target molecules labeled with FITC.Fluorescein isothiocyanate (FITC) is a commonly used fluorescent dye in biology and medicine, making this antibody suitable for a wide range of research areas, including cell biology, immunology, and molecular biology.
By specifically recognizing FITC-labeled molecules, this antibody facilitates the detection and quantification of target proteins in samples, providing valuable insights into cellular processes and interactions.With its high reactivity and reliability, the Anti-Fluorescein Isothiocyanate (FITC) Antibody (CAB20385) is a versatile tool for researchers seeking to advance their understanding of fluorescently labeled molecules and their roles in biological systems. Its broad applicability and consistent performance make it an essential component of any laboratory working with fluorescence-based assays and experiments.
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
The FITC rabbit polyclonal antibody (CAB20385) are tested in Western blot against FITC conjugated Goat IgG,TRITC conjugated Goat IgG and unconjugated Goat IgGSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: Refer to figures.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
