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Fluorescein FITC Monoclonal Antibody (CAB20797)

SKU:
CAB20797
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Donkey
Isotype:
Horseradish peroxidase conjugated IgG
€179 - €469
Frequently bought together:

Description

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Fluorescein FITC Monoclonal Antibody (CAB20797)

The Anti-Fluorescein FITC Antibody (CAB20797) is a valuable tool for researchers working with fluorescein-labeled molecules in various applications. This antibody, raised in rabbits, exhibits high reactivity and specificity for fluorescein, making it ideal for use in immunofluorescence assays and other fluorescence-based techniques. By binding specifically to fluorescein, this antibody enables the detection and visualization of fluorescein-labeled molecules in cell and tissue samples, facilitating studies in fields such as immunology, molecular biology, and cell signaling.

This antibody is an essential component for researchers looking to accurately detect and quantify fluorescein-labeled targets, offering reliable and reproducible results for their experiments.

Product Name:Fluorescein FITC Monoclonal Antibody
SKU:CAB20797
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Species independent
Immunogen:FITC
Sequence:FITC con juga ted to K LH
Tested Applications:WB ELISA
Recommended Dilution:ELISA,1:500 - 1:2000 WB,1:1000 - 1:2000
Conjugate:Unconjugated
Observed MW:25kDa/55kDa

Fluorescein isothiocyanate (FITC) labeling is a common technique with a wide range of? applications because it reacts quickly with amines and due to its high quantum efficacy. Due to its high molecular absorptivity, using FITC labels is preferred over conventional colorimetric labels and radio labels because fluorophores like FITC are bright, easier to work with, and don’t require special waste handling. Proteins, substrates, peptide hormones, and antibodies? labeled by FITC can be used as probes in flow cytometry, enzyme kinetics, and immunocytochemistry, as well as in the detection of receptors on the surface of? the target cells.5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.6-FAM (6-Carboxyfluorescein) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

Purification Method:Affinity purification
Clone Number:ARC51071
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageImmobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 μg/mL (25 μl/well), of which EC50ranges are 0.27-0.54 ng/mL, 0.18-0.31 ng/mL and 0.12-0.39 ng/mL , can bind anti 6-Fam/ 5-Fam/ FITC mAb (CAB20797), respectively.

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