The Anti-Fibrillarin Antibody (CAB0850) is a valuable tool for researchers studying fibrillarin, a key protein involved in ribosome biogenesis and RNA processing. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been validated for use in various applications, including Western blotting.Fibrillarin is a nucleolar protein essential for the synthesis of ribosomal RNA and the processing of small nucleolar RNAs. It plays a crucial role in maintaining cell growth and proliferation, making it a target of interest in cancer research and other diseases characterized by abnormal cell growth.
By specifically binding to fibrillarin, this antibody allows for the detection and analysis of the protein in different cell types, providing valuable insights into its function and potential implications in various physiological and pathological processes. Researchers in the fields of molecular biology, cell biology, and oncology will find this antibody to be an essential tool for their studies into the role of fibrillarin in health and disease.
Product Name:
Fibrillarin/U3 RNP Rabbit Monoclonal Antibody
SKU:
CAB0850
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 222-321 of human Fibrillarin/U3 RNP (P22087).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
FIB; FLRN; Nop1; RNU3IP1; Fibrillarin/U3 RNP
Positive Sample:
293T,Hep G2,Jurkat,Mouse spleen,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus.
Calculated MW:
34kDa
Observed MW:
37kDa
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
Purification Method:
Affinity purification
Gene ID:
2091
Clone Number:
ARC0506
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Fibrillarin/U3 RNP Rabbit mAb (CAB0850) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
