The Ezrin, Radixin, Moesin (ERM) Polyclonal Antibody (CAB21093) is a valuable tool for researchers studying the Ezrin, Radixin, and Moesin proteins, which play essential roles in cell structure and function. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.The ERM family of proteins are known for their ability to link the actin cytoskeleton to the plasma membrane, important for maintaining cell shape, adhesion, and motility. Dysregulation of Ezrin, Radixin, and Moesin has been implicated in various diseases, including cancer, metastasis, and neurological disorders.
By targeting the ERM proteins, researchers can study their involvement in cell signaling pathways, cytoskeletal dynamics, and disease progression. The Ezrin, Radixin, Moesin Polyclonal Antibody provides a means of detecting and analyzing these proteins in a variety of cell types, making it a valuable tool for investigations in cell biology, cancer research, and other fields. Understanding the functions and regulation of the ERM proteins is essential for advancing our knowledge of cellular processes and disease mechanisms.
Product Name:
Ezrin/Radixin/Moesin Polyclonal Antibody
SKU:
CAB21093
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 487-586 of human Ezrin/Radixin/Moesin (NP_003370.2).
actin cytoskeleton, apical plasma membrane, basolateral plasma membrane, cell projection, ciliary basal body, cortical cytoskeleton, cytoplasm, cytoplasmic side of apical plasma membrane, cytosol, endosome, extracellular exosome, extracellular space, fibrillar center, filopodium, focal adhesion, immunological synapse, microvillus membrane, perinuclear region of cytoplasm, plasma membrane, plasma membrane raft, ruffle membrane
Calculated MW:
69kDa/68kDa/67kDa
Observed MW:
76kDa/80kDa
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
Purification Method:
Affinity purification
Gene ID:
7430/5962/4478
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Ezrin/Radixin/Moesin antibody (CAB21093) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.8s.
