The Anti-EV71 3C Antibody (CAB20687) is a powerful tool for researchers studying Enterovirus 71 (EV71), a common cause of hand-foot-and-mouth disease in children. This polyclonal antibody, produced in rabbits, is highly specific to EV71 3C protein and is validated for use in various applications, including immunofluorescence and ELISA.EV71 is a picornavirus that can cause severe neurological complications and even death in young children. The 3C protein is a key virulence factor involved in viral replication and pathogenesis, making it a valuable target for therapeutic interventions.
By using the Anti-EV71 3C Antibody, researchers can study the structure and function of the 3C protein, as well as develop diagnostic tools and potential antiviral therapies for EV71 infection.Overall, the Anti-EV71 3C Antibody is an essential tool for advancing our understanding of EV71 pathogenesis and developing strategies to combat this important public health threat.
Product Name:
EV71 3C Polyclonal Antibody
SKU:
CAB20687
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Other (Wide Range Predicted)
Immunogen:
Recombinant protein of human EV71 3C.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
HeLa
Conjugate:
Unconjugated
Observed MW:
20kDa
Enterovirus 71 (EV71) is the main pathogen of the hand, foot, and mouth disease. It was firstly isolated from sputum specimens of infants with central nervous system diseases in California in 1969, and has been repeatedly reported in various parts of the world, especially in the Asia-Pacific region. Infection with EV71 can lead tosevere neurological complications such as encephalitis, acuteflaccid paralysis, and pulmonary edema in children (1). EV71 belongs to the genus Enterovirus within the Picorna viridae family, which is a non-enveloped virus with a singlepositive-stranded RNA genome.EV71 3C protein is a 183 amino acid cysteine protease that can cleave most structural and non-structural proteins of EV71. Based on the analysis and understanding of EV71 3C protease, it is helpful to study and treat diseases caused by EV71 virus infection. The EV71 3C protease promotes virus replication by cleaving EV71 synthesis or host proteins. Moreover, EV71 3C protease inhibits the innate immune system and causes apoptosis.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using EV71 3C Rabbit pAb (CAB20687) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
