The Eph Receptor B3 (EphB3) Polyclonal Antibody (CAB19229) is a valuable tool for research involving EphB3, a member of the Eph receptor family known for its role in cell signaling and development. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various applications such as Western blot and immunohistochemistry.EphB3 is a transmembrane receptor tyrosine kinase that plays a crucial role in regulating cell adhesion, migration, and axon guidance. Dysregulation of EphB3 signaling has been implicated in various diseases, including cancer, neurological disorders, and cardiovascular conditions.
By targeting EphB3 with this antibody, researchers can explore its function in different cellular processes and gain insights into potential therapeutic strategies for related diseases.Overall, the Eph Receptor B3 Polyclonal Antibody is a reliable tool for studying EphB3 protein expression and localization in diverse biological contexts, making it an essential component for researchers interested in understanding the complexities of Eph receptor signaling pathways.
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Eph receptor B3 (EPHB3) (EPHB3) (P54753).
Sequence:
SELA WTSH PESG WEEV SGYD EAMN PIRT YQVC NVRE SSQN NWLR TGFI WRRD VQRV YVEL KFTV RDCN SIPN IPGS CKET FNLF YYEA DSDV ASAS SPFW M
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
EK2; ETK2; HEK2; TYRO6; Eph receptor B3 (EPHB3)
Positive Sample:
HeLa,HT-29,SH-SY5Y,Mouse brain,Rat brain
Conjugate:
Unmodified
Cellular Localization:
Cell membrane, Cell projection, Single-pass type I membrane protein, dendrite.
Calculated MW:
110kDa
Observed MW:
110kDa
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members.
Purification Method:
Affinity purification
Gene ID:
2049
Clone Number:
ARC2384
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Eph receptor B3 (EPHB3) (EPHB3) antibody (CAB19229) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
