The eNOS Polyclonal Antibody (CAB20985) is a valuable tool for researchers studying endothelial nitric oxide synthase (eNOS), an important enzyme involved in the production of nitric oxide and regulation of vascular tone. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot and immunohistochemistry applications.eNOS, also known as NOS3, plays a key role in the cardiovascular system by modulating blood pressure, vascular tone, and blood flow. Dysregulation of eNOS activity has been implicated in various cardiovascular diseases, making it a target of interest for drug development and therapeutic interventions.
By targeting eNOS, researchers can gain insights into vascular function, endothelial dysfunction, and the pathophysiology of cardiovascular disorders.With its high reactivity and specificity, the eNOS Polyclonal Antibody (CAB20985) offers researchers a reliable tool for detecting and studying eNOS expression in various tissues and cell types. Whether investigating the role of eNOS in cardiovascular health, endothelial function, or vascular pathologies, this antibody provides a valuable resource for advancing research in cardiovascular biology and vascular medicine.
Product Name:
eNOS Monoclonal Antibody
SKU:
CAB20985
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1110 of human eNOS (NP_000594.2).
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4846
Clone Number:
ARC51618
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using eNOS antibody (CAB20985) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
