null

EIF4A1/EIF4A2/EIF4A3 Rabbit Polyclonal Antibody (CAB20251)

SKU:
CAB20251
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:EIF4A1/EIF4A2/EIF4A3 Rabbit Polyclonal Antibody
SKU:CAB20251
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A (NP_001407.1).
Sequence:MSAS QDSR SRDN GPDG MEPE GVIE SNWN EIVD SFDD MNLS ESLL RGIY AYGF EKPS AIQQ RAIL PCIK GYDV IAQA QSGT GKTA TFAI SILQ QIEL DLKA
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Positive Sample:293T,HeLa,HepG2,MCF7,C2C12,Mouse brain,Rat brain
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, eukaryotic translation initiation factor 4F complex, extracellular exosome
Observed MW:46kDa

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Purification Method:Affinity purification
Gene ID:1973/1974/9775
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using eIF4A antibody (CAB20251) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Related Products