The EIF4A1/EIF4A2/EIF4A3 Polyclonal Antibody (CAB20251) is a valuable tool for researchers studying the eukaryotic initiation factor 4A (EIF4A) family of proteins. This antibody, derived from rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot applications. The EIF4A family of proteins, including EIF4A1, EIF4A2, and EIF4A3, are essential for mRNA translation and play a critical role in protein synthesis. Dysregulation of EIF4A proteins has been implicated in various diseases, including cancer, making them an important target for research.
The EIF4A1/EIF4A2/EIF4A3 antibody facilitates the detection and analysis of these proteins in different cell types, providing valuable insights into their function and regulation. With its applications in immunology and cancer research, this antibody is a key resource for scientists looking to elucidate the role of EIF4A proteins in health and disease.
Product Name:
EIF4A1/EIF4A2/EIF4A3 Rabbit Polyclonal Antibody
SKU:
CAB20251
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A (NP_001407.1).
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Purification Method:
Affinity purification
Gene ID:
1973/1974/9775
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using eIF4A antibody (CAB20251) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
