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eIF2A Rabbit Monoclonal Antibody (CAB9709)

SKU:
CAB9709
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CDA02
EIF-2A
MST089
MSTP004
MSTP089
Research Area:
Metabolism
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:eIF2A Rabbit Monoclonal Antibody
SKU:CAB9709
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 450-550 of human eIF2A (Q9BY44).
Sequence:ALRN KPIT NSKL HEEE PPQN MKPQ SGND KPLS KTAL KNQR KHEA KKAA KQEA RSDK SPDL APTP APQS TPRN TVSQ SISG DPEI DKKI KNLK KKLK AIEQ L
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:CDA02; EIF-2A; MST089; MSTP004; MSTP089; eIF2A
Positive Sample:BxPC-3,Mouse liver,Rat lung
Conjugate:Unconjugated
Cellular Localization:blood microparticle, cytoplasm, eukaryotic translation initiation factor 2 complex, extracellular space
Calculated MW:65kDa
Observed MW:60kDa

This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:83939
Clone Number:ARC1712
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using eIF2A Rabbit mAb (CAB9709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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