The eIF2A Polyclonal Antibody (CAB9709) is a valuable tool for researchers studying eIF2A, a crucial protein involved in the regulation of translation initiation. This antibody, produced in rabbits, exhibits high specificity for human samples and has been rigorously validated for Western blot applications.eIF2A is an essential factor in protein synthesis, controlling the initiation of translation in response to various cellular stresses. Dysregulation of eIF2A activity has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and viral infections.
By targeting eIF2A, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets. The eIF2A Polyclonal Antibody enables the detection and analysis of eIF2A in diverse cell types, making it a valuable tool for investigations in molecular biology, cancer research, and infectious diseases.
Product Name:
eIF2A Rabbit Monoclonal Antibody
SKU:
CAB9709
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 450-550 of human eIF2A (Q9BY44).
This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
83939
Clone Number:
ARC1712
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using eIF2A Rabbit mAb (CAB9709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
