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DNMT1 Polyclonal Antibody (CAB15050)

SKU:
CAB15050
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:DNMT1 Polyclonal Antibody
SKU:CAB15050
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1).
Sequence:MPAR TAPA RVPT LAVP AISL PDDV RRRL KDLE RDSL TEKE CVKE KLNL LHEF LQTE IKNQ LCDL ETKL RKEE LSEE GYLA KVKS LLNK DLSL ENGA HAYN REVN GRLE NGNQ ARSE ARRV GMAD
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNMT1
Positive Sample:HeLa,293T,Jurkat,U-87MG,Mouse testis
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:183kDa
Observed MW:200kDa

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:1786
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DNMT1 Rabbit pAb (CAB15050) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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