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Anti-DNA Ligase I Antibody (CAB9301)

SKU:
CAB9301
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€139 - €419
Frequently bought together:

Description

Anti-DNA Ligase I Antibody (CAB9301)

The DNA Ligase I Polyclonal Antibody (CAB9301) is a high-quality research tool designed for studying DNA ligase I, an essential enzyme involved in DNA replication and repair processes. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. By binding specifically to DNA ligase I, researchers can accurately detect and analyze the enzyme's expression levels in various cell types.DNA ligase I plays a crucial role in maintaining the integrity of the genome by sealing nicks in DNA strands during DNA synthesis and repair.

Dysregulation of DNA ligase I function has been implicated in various diseases, including cancer and genetic disorders. Studying the activity of this enzyme provides valuable insight into cellular processes and potential therapeutic targets for disease treatment.The DNA Ligase I Polyclonal Antibody is a valuable tool for researchers investigating DNA repair mechanisms, genome stability, and the development of novel cancer therapies. Its high specificity and reliability make it an ideal choice for immunology and oncology studies.

Product Name:DNA Ligase I Rabbit mAb
Product Code:CAB9301
Size:20uL, 50uL, 100uL
Applications:WB, IHC, IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human DNA Ligase I
Applications:WB, IHC, IF
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat
Immunogen:A synthesized peptide derived from human DNA Ligase I
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3978
Uniprot:P18858
Calculated MW:140kDa
Observed MW:130KDa
UniProt Protein Function:LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
UniProt Protein Details:

Protein type:Ligase; EC 6.5.1.1; DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.2-q13.3

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; chromosome; nucleus

Molecular Function:DNA binding; DNA ligase activity; metal ion binding; DNA ligase (ATP) activity; ATP binding

Biological Process: anatomical structure morphogenesis; mismatch repair; V(D)J recombination; DNA strand elongation during DNA replication; DNA repair; lagging strand elongation; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; response to hydrogen peroxide; base-excision repair; nucleotide-excision repair; cell division; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; DNA ligation during DNA repair; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; DNA metabolic process

NCBI Summary:This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:P18858
NCBI GenInfo Identifier:118773
NCBI Gene ID:3978
NCBI Accession:P18858.1
UniProt Related Accession:P18858
Molecular Weight:Predicted Band Size: 102kDa
NCBI Full Name:DNA ligase 1
NCBI Synonym Full Names:DNA ligase 1
NCBI Official Symbol:LIG1  
NCBI Protein Information:DNA ligase 1
UniProt Protein Name:DNA ligase 1
UniProt Synonym Protein Names:DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1
UniProt Gene Name:LIG1  
UniProt Entry Name:DNLI1_HUMAN

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