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DBT Rabbit Polyclonal Antibody (CAB20381)

SKU:
CAB20381
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
E2
E2B
BCATE2
BCKADE2
BCKAD-E2
BCKDH-E2
BCOADC-E2
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:DBT Rabbit Polyclonal Antibody
SKU:CAB20381
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3).
Sequence:SRNA GKLI CVRY FQTC GNVH VLKP NYVC FFGY PSFK YSHP HHFL KTTA ALRG QVVQ FKLS DIGE GIRE VTVK EWYV KEGD TVSQ FDSI CEVQ SDKA SVTI TSRY DGVI KKLY YNLD DIAY VGKP LVDI ETEA LKDS EEDV VETP AVSH DEHT HQEI KGRK TLAT PAVR RLAM ENNI KLSE VVGS GKDG RILK EDIL NYLE KQTG AILP PSPK VEIM PPPP KPKD MTVP ILVS KPPV F
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCKDH-E2; BCOADC-E2; DBT
Positive Sample:293T,HepG2,A375,Mouse lung,Mouse brain,Rat liver,Rat brain
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, microtubule cytoskeleton, mitochondrial alpha-ketoglutarate dehydrogenase complex, mitochondrial matrix, mitochondrial nucleoid, mitochondrion
Calculated MW:53kDa
Observed MW:53kDa

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Purification Method:Affinity purification
Gene ID:1629
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DBT Rabbit pAb (CAB20381) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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