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Cytokeratin 18 (KRT18) Rabbit Monoclonal Antibody (CAB19778)

SKU:
CAB19778
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CK18
K18
Krt1-18
Research Area:
Cell Cycle
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Cytokeratin 18 (KRT18) Rabbit Monoclonal Antibody
SKU:CAB19778
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-430 of human Cytokeratin 18 (KRT18) (P05783).
Sequence:ADIR AQYD ELAR KNRE ELDK YWSQ QIEE STTV VTTQ SAEV GAAE TTLT ELRR TVQS LEID LDSM RNLK ASLE NSLR EVEA RYAL QMEQ LNGI LLHL ESEL AQTR AEGQ RQAQ EYEA LLNI KVKL EAEI ATYR RLLE DGED FNLG DALD SSNS MQTI QKTT TRRI VDGK VVSE TNDT KVLR H
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:K18; CK-18; CYK18; Cytokeratin 18 (KRT18)
Positive Sample:HeLa,HepG2,A-431
Conjugate:Unconjugated
Cellular Localization:adherens junction, cytoplasm, cytoskeleton, cytosol, extracellular exosome, microtubule organizing center, nuclear matrix, nucleolus, perinuclear region of cytoplasm
Calculated MW:48kDa
Observed MW:48kDa

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Purification Method:Affinity purification
Gene ID:3875
Clone Number:ARC2303
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Cytokeratin 18 (KRT18) Rabbit mAb (CAB19778) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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