The Anti-Cytokeratin 12 (KRT12) Antibody (CAB19284) is a valuable tool for research involving the cytokeratin 12 protein, which plays a crucial role in epithelial cell differentiation and maintenance. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as immunohistochemistry and Western blotting.Cytokeratin 12 is a member of the intermediate filament family of proteins that are essential for maintaining the structural integrity of epithelial cells. Aberrant expression of cytokeratin 12 has been associated with various diseases, including ocular disorders and certain types of cancer.
Therefore, the Anti-Cytokeratin 12 Antibody is a valuable tool for studying the expression and function of this protein in different cell types and disease states.Researchers in the fields of ophthalmology, oncology, and cell biology can benefit from using this antibody to investigate the role of cytokeratin 12 in normal cellular processes and pathological conditions. Its specificity and reliability make it an essential reagent for studies aiming to understand the molecular mechanisms underlying epithelial cell biology and disease progression.
Product Name:
Cytokeratin 12 (KRT12) Rabbit Monoclonal Antibody
SKU:
CAB19284
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-494 of human Cytokeratin 12 (KRT12) (NP_000214.1).
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.
Purification Method:
Affinity purification
Gene ID:
3859
Clone Number:
ARC2465
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat eye, using Cytokeratin 12 (KRT12) Rabbit mAb (CAB19284) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
