The Anti-Cystatin B Antibody (CAB20886) is a valuable tool for research into the protein Cystatin B, which plays a crucial role in regulating protease activity and maintaining cellular homeostasis. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in various applications such as Western blotting and immunofluorescence.Cystatin B is a member of the cystatin family of protease inhibitors and is involved in inhibiting lysosomal cathepsins, enzymes that break down proteins. Dysregulation of Cystatin B has been implicated in various diseases, including neurodegenerative disorders and cancer.
By targeting Cystatin B with this antibody, researchers can gain insights into the function and dysregulation of this protein in different cellular contexts.Moreover, the Anti-Cystatin B Antibody is a valuable tool for investigating potential therapeutic strategies targeting Cystatin B in diseases where its dysregulation plays a role. By understanding the mechanisms by which Cystatin B contributes to disease pathogenesis, researchers can develop targeted therapies aimed at restoring cellular homeostasis and potentially treating these disorders effectively.
Product Name:
Cystatin B Monoclonal Antibody
SKU:
CAB20886
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-98 of human Cystatin B (NP_000091.1).
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.
Purification Method:
Affinity purification
Gene ID:
1476
Clone Number:
ARC2833
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cystatin B antibody (CAB20886) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
