The Anti-Cyclin A1/A2 Antibody (CAB2635) is a highly specific and sensitive tool for studying the expression and function of cyclin A1 and A2 proteins. Cyclin A1 and A2 are key regulators of cell cycle progression, playing essential roles in cell division and proliferation. This antibody, produced for research purposes, is optimized for detecting cyclin A1 and A2 in human samples, making it ideal for investigating the role of these proteins in various biological processes.Cyclin A1 and A2 are known to be dysregulated in various diseases, including cancer, making them attractive targets for therapeutic interventions.
By using the Anti-Cyclin A1/A2 Antibody, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially discover new treatment strategies. The antibody is validated for use in various experimental techniques, such as Western blotting, immunofluorescence, and immunohistochemistry, allowing for versatile applications in the fields of oncology, cell biology, and developmental biology. In summary, the Anti-Cyclin A1/A2 Antibody (CAB2635) is a valuable tool for researchers interested in elucidating the roles of cyclin A1 and A2 in health and disease. Its high specificity
Product Name:
Cyclin A1/A2 Rabbit Monoclonal Antibody
SKU:
CAB2635
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Cyclin A1/A2 (P20248).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
CCN1; CCNA; CT146; Cyclin A1/A2
Positive Sample:
HCT116,Jurkat,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, nucleus
Calculated MW:
52kDa/49kDa
Observed MW:
52kDa/55kDa
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
890/8900
Clone Number:
ARC2642
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cyclin A1/A2 Rabbit mAb (CAB2635) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
