Cyclin A1/A2 Rabbit Monoclonal Antibody (CAB2635)
- SKU:
- CAB2635
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Product Name: | Cyclin A1/A2 Rabbit Monoclonal Antibody |
| SKU: | CAB2635 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Cyclin A1/A2 (P20248). |
| Sequence: | RGKL QLVG TAAM LLAS KFEE IYPP EVAE FVYI TDDT YTKK QVLR MEHL VLKV LTFD LAAP TVNQ FLTQ YFLH QQPA NCKV ESLA MFLG ELSL IDAD PYLK YLPS VIAG AAFH LALY TVTG QSWP ESLI RKTG YTLE SLKP CLMD LHQT YLKA PQHA QQSI REKY KNSK YHGV SLLN PPET LNL |
| Tested Applications: | WB IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells |
| Synonyms: | CCN1; CCNA; CT146; Cyclin A1/A2 |
| Positive Sample: | HCT116,Jurkat,Mouse thymus |
| Conjugate: | Unconjugated |
| Cellular Localization: | cytoplasm, nucleus |
| Calculated MW: | 52kDa/49kDa |
| Observed MW: | 52kDa/55kDa |
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 890/8900 |
| Clone Number: | ARC2642 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using Cyclin A1/A2 Rabbit mAb (CAB2635) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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