The Cytochrome b5 Reductase 3 Polyclonal Antibody (CAB20892) is a valuable tool for researchers studying Cytochrome b5 Reductase 3 (CYB5R3), an essential enzyme involved in electron transfer processes in cells. This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting CYB5R3 in human samples, making it suitable for use in Western blot applications.CYB5R3 is a key player in various cellular processes, including lipid metabolism, drug metabolism, and electron transport chain activity. Dysregulation of CYB5R3 has been linked to diseases such as hereditary methemoglobinemia and cancer, highlighting its importance in physiological and pathological conditions.
By targeting CYB5R3 with this antibody, researchers can delve deeper into its functions and explore potential therapeutic implications in these disease contexts.With its ability to specifically recognize CYB5R3, this polyclonal antibody is a valuable tool for furthering research in biochemistry, cell biology, and disease mechanisms. Its versatility in detecting CYB5R3 in different cell types makes it a versatile asset for investigators studying various aspects of cellular function and disease pathways.
Product Name:
CYB5R3 Monoclonal Antibody
SKU:
CAB20892
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CYB5R3 (P00387).
This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias.
Purification Method:
Affinity purification
Gene ID:
1727
Clone Number:
ARC2849
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various lysates, using CYB5R3 antibody (CAB20892) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
