Creatine kinase B type (CKB) Monoclonal Antibody (CAB4907)
The Creatine kinase B type (CKB) Monoclonal Antibody (CAB4907) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. RRID AB_2863385 Gene ID 1152 Swiss Prot Synonym BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29; Creatine kinase B type (CKB)
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Creatine kinase B type (CKB) Monoclonal Antibody
SKU:
CAB4907
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1990
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BCK, B-CK, CKBB, CPK-B, HEL-211, HEL-S-29, Creatine kinase B type (CKB)
Positive Sample:
HeLa, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Cytosol, Mitochondrion, Cell Membrane.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. RRID AB_2863385 Gene ID 1152 Swiss Prot Synonym BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29; Creatine kinase B type (CKB)
Purification Method:
Affinity purification
Gene ID:
1152
RRID:
AB_2863385
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Creatine kinase B type (CKB) Rabbit mAb (CAB4907) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using Creatine kinase B type (CKB) Rabbit mAb (CAB4907) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
