The Anti-COLD1 Antibody (CAB20713) is a polyclonal antibody designed for research involving COLD1, a novel transmembrane protein that plays a crucial role in cold stress response in plants. This antibody is raised in rabbits and is highly reactive with plant samples, making it a valuable tool for studying the molecular mechanisms involved in cold stress tolerance.COLD1, also known as Cold-regulated Protein 1, is involved in the regulation of cold response pathways in plants, including the expression of cold-responsive genes and the activation of cold tolerance mechanisms.
Understanding the function of COLD1 is essential for developing strategies to enhance cold stress tolerance in crops, which is important for ensuring food security in a changing climate.The Anti-COLD1 Antibody binds specifically to the COLD1 protein, allowing for the detection and analysis of COLD1 expression in plant tissues. It has been validated for use in various research applications, including Western blotting and immunohistochemistry, making it an essential tool for researchers studying plant stress responses and cold tolerance mechanisms.
Product Name:
Cold1 Polyclonal Antibody
SKU:
CAB20713
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Oryza sativa
Immunogen:
Recombinant protein of Oryza sativa Cold1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
COLD1; OsJ_16035; Cold1
Positive Sample:
leaves,stems,spikes
Conjugate:
Unconjugated
Cellular Localization:
plasma membrane
Calculated MW:
53kDa
Observed MW:
53kDa
Involved in chilling tolerance. Interacts with the G-protein alpha subunit GPA1 to activate the calcium channel for sensing low temperature and to accelerate G-protein GTPase activity.
Purification Method:
Affinity purification
Gene ID:
4336878
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Cold1 Rabbit pAb (CAB20713) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
