The ClpX Polyclonal Antibody (CAB20871) is a vital tool for researchers studying the ClpX protein, an ATP-dependent protein unfolding and translocating chaperone involved in protein quality control. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications.ClpX plays a crucial role in protein degradation and turnover within cells, making it an essential component of cellular quality control mechanisms. By targeting the ClpX protein, researchers can investigate its function in various cellular processes, including protein folding, degradation, and maintenance of cellular homeostasis.
Furthermore, understanding the role of ClpX in cellular pathways can provide valuable insights into disease mechanisms, particularly in conditions where protein misfolding and aggregation play a role, such as neurodegenerative disorders. By utilizing the ClpX Polyclonal Antibody, researchers can further elucidate the intricate workings of cellular quality control systems and potentially uncover new therapeutic targets for the treatment of protein misfolding diseases.
Product Name:
CLPX Monoclonal Antibody
SKU:
CAB20871
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 534-633 of human CLPX (O76031).
The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10845
Clone Number:
ARC2812
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CLPX antibody (CAB20871) at1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
