The Anti-Cleaved PARP (p25) Antibody (CAB19612) is a valuable tool for researchers studying programmed cell death processes, such as apoptosis. This antibody specifically targets the cleaved PARP (poly(ADP-ribose) polymerase) p25 protein, a well-known marker of apoptosis, making it an essential reagent for investigating cell death pathways.Raised in rabbits, this polyclonal antibody exhibits high specificity and sensitivity in detecting cleaved PARP p25 in human samples. It has been rigorously validated for use in Western blot applications, providing reliable results for researchers studying apoptosis in various cell types. The cleaved PARP p25 protein is a key player in the signaling cascade of apoptosis, serving as a substrate for caspases and indicating the activation of programmed cell death processes.
By targeting this specific protein fragment, researchers can gain insights into the mechanisms of apoptosis and assess the efficacy of anti-cancer therapies that induce cell death in tumor cells.Overall, the Anti-Cleaved PARP (p25) Antibody is an essential tool for investigating apoptotic pathways and understanding the molecular mechanisms underlying cell death processes. Its application in cancer research, drug development, and other fields makes it a valuable asset for scientists studying programmed cell death.
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Purification Method:
Affinity purification
Gene ID:
142
Clone Number:
ARC0091
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cleaved PARP1 p25 Rabbit mAb (CAB19612) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
