The Anti-CHMP6 Antibody (CAB4975) is a valuable tool for researchers studying the CHMP6 protein, a key player in the endosomal sorting complex required for transport (ESCRT) pathway. This polyclonal antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.CHMP6 is involved in the formation of multivesicular bodies (MVBs) and the sorting of endocytic cargo for degradation or recycling. Dysregulation of the ESCRT machinery, including CHMP6, has been linked to various diseases, making it a target of interest in studies on cancer, viral infections, and neurodegenerative disorders.
By specifically binding to CHMP6, this antibody allows for the detection and analysis of this important protein in different cell types, enabling researchers to further understand its role in cellular processes and disease pathology. Its high reactivity and specificity make it an ideal tool for immunology and cell biology research.
Product Name:
CHMP6 Rabbit Polyclonal Antibody
SKU:
CAB4975
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 111-200 of human CHMP6 (NP_078867.2).
Sequence:
LNKM HQVM SIEE VERI LDET QEAV EYQR QIDE LLAG SFTQ EDED AILE ELSA ITQE QIEL PEVP SEPL PEKI PENV PVKA RPRQ AELV AA
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
VPS20; CHMP6
Positive Sample:
U2OS,PC-3,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Endomembrane system, Endosome membrane, Late endosome membrane, Lipid-anchor, Membrane.
Calculated MW:
23kDa
Observed MW:
30kDa
This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein family. Proteins in this family are part of the ESCRT-III (endosomal sorting complex required for transport III) which degrades surface receptors, and in biosynthesis of endosomes.
Purification Method:
Affinity purification
Gene ID:
79643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CHMP6 antibody (CAB4975) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
