CDKN1A Rabbit Polyclonal Antibody (CAB20471)
- SKU:
- CAB20471
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- CI
- P2
- SD
- CAP
- CDK
- P21
- Waf
- mda
- CDKI
- CIP1
- Cdkn
- SDI1
- Waf1
- mda6
- p21C
- p21W
- CAP20
- Cdkn1
- p21WAF
- p21Cip1
Description
| Product Name: | CDKN1A Rabbit Polyclonal Antibody |
| SKU: | CAB20471 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-159 of mouse CDKN1A (NP_031695.1). |
| Sequence: | MSNP GDVR PVPH RSKV CRCL FGPV DSEQ LRRD CDAL MAGC LQEA RERW NFDF VTET PLEG NFVW ERVR SLGL PKVY LSPG SRSR DDLG GDKR PSTS SALL QGPA PEDH VALS LSCT LVSE RPED SPGG PGTS QGRK RRQT SLTD FYHS KRRL VFCK RKP |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | P21; CDKI; CIP1; SDI1; Waf1; mda6; CAP20; Cdkn1; p21WAF; p21Cip1; CDKN1A |
| Positive Sample: | Mouse large intestine,Mouse spleen,Rat heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | cytoplasm, cytosol, nuclear body, nucleolus, nucleoplasm, nucleus, perinuclear region of cytoplasm |
| Calculated MW: | 18kDa |
| Observed MW: | 21kDa |
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at the G1 pahse. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Alternative splicing results in multiple transcript variants.
| Purification Method: | Affinity purification |
| Gene ID: | 12575 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using CDKN1A antibody (CAB20471) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s. |
Related Products
CDKN1A/p21CIP1 Rabbit Polyclonal Antibody (CAB11454)
CDKN1A/p21CIP1 Rabbit Polyclonal Antibody (CAB1483)
CDKN1A/p21CIP1 Polyclonal Antibody (CAB21323)
CDKN1A/p21CIP1 Polyclonal Antibody (CAB21772)
HNRRabbit Polyclonal Antibody Rabbit Polyclonal Antibody (CAB17497)
