The Anti-CDC42 Antibody (CAB19028) is a valuable tool for researchers studying the CDC42 protein, a key regulator of cell signaling pathways involved in processes such as cell division, migration, and invasion. This antibody is produced in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of CDC42 protein levels in various cell types.CDC42 is known for its role in cytoskeletal dynamics and cell polarity, making it a crucial player in processes like cell motility and adhesion. Dysregulation of CDC42 has been linked to diseases such as cancer, where abnormal cell migration and invasion contribute to tumor progression.
By targeting CDC42 with this antibody, researchers can further investigate its involvement in cancer development and potentially identify new therapeutic targets for treatment.In addition to cancer research, the Anti-CDC42 Antibody can also be used in studies related to other cellular processes that rely on CDC42 activity, such as neuronal development, immune response, and embryogenesis. Its high reactivity with human samples makes it a versatile tool for understanding the functions of CDC42 in various physiological and pathological contexts.
Product Name:
CDC42 Rabbit Monoclonal Antibody
SKU:
CAB19028
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-191 of human CDC42 (P60953).
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20.
Purification Method:
Affinity purification
Gene ID:
998
Clone Number:
ARC0484
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CDC42 antibody (CAB19028) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
