The CDC34 Polyclonal Antibody (CAB9772) is a valuable tool for researchers studying CDC34, a crucial protein involved in the regulation of cell cycle progression and DNA repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the CDC34 protein, this antibody allows for its detection and analysis in various cell types, making it ideal for research in the fields of molecular biology and cancer biology.
CDC34 is a key player in the ubiquitin-proteasome system, where it functions as an E2 ubiquitin-conjugating enzyme. The intricate role of CDC34 in protein degradation pathways and DNA damage response makes it a promising target for investigating diseases such as cancer and neurodegenerative disorders. Understanding the mechanisms by which CDC34 operates is essential for developing therapeutic strategies that target aberrant cell cycle regulation and DNA repair pathways implicated in various diseases.
Product Name:
CDC34 Rabbit Monoclonal Antibody
SKU:
CAB9772
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-236 of human CDC34 (P49427).
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Purification Method:
Affinity purification
Gene ID:
997
Clone Number:
ARC1742
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CDC34 Rabbit mAb (CAB9772) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
