CDC27 Rabbit Monoclonal Antibody (CAB3333)
- SKU:
- CAB3333
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- ANAPC3
- APC3
- CDC27Hs
- D0S1430E
- D17S978E
- H-NUC
- HNUC
- NUC2
Description
Product Name: | CDC27 Rabbit Monoclonal Antibody |
SKU: | CAB3333 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 702-824 of human CDC27 (P30260). |
Sequence: | NPLC KFHR ASVL FANE KYKS ALQE LEEL KQIV PKES LVYF LIGK VYKK LGQT HLAL MNFS WAMD LDPK GANN QIKE AIDK RYLP DDEE PITQ EEQI MGTD ESQE SSMT DADD TQLH AAES DEF |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 |
Synonyms: | APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E; CDC27 |
Positive Sample: | DU 145,Mouse testis |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 92kDa |
Observed MW: | 100kDa |
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.
Purification Method: | Affinity purification |
Gene ID: | 996 |
Clone Number: | ARC1947 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |