The Anti-CD47 Recombinant Rabbit Monoclonal Antibody (HDAB0203) is a powerful tool for researchers studying the CD47 protein, a key molecule involved in immune regulation and immune evasion in cancer cells. This antibody, generated in rabbits, is highly specific to human CD47 and has been validated for use in various applications, including immunohistochemistry, immunofluorescence, and flow cytometry.CD47 is known to interact with signal regulatory protein alpha (SIRPα) on macrophages, leading to the "don't eat me" signal that allows cancer cells to evade the immune system. By targeting CD47 with this monoclonal antibody, researchers can investigate the role of CD47 in immune evasion and potentially develop novel immunotherapies for cancer treatment.
The Anti-CD47 Recombinant Rabbit Monoclonal Antibody (HDAB0203) provides a reliable tool for studying the function of CD47 in cancer and other diseases where immune evasion is a factor. Its high specificity and sensitivity make it a valuable resource for researchers looking to uncover the mechanisms behind immune regulation and develop targeted therapies for cancer and other immune-related disorders.
SKU:
HDAB0203
Size:
100 µg
Clonality:
Monoclonal
Clone:
DM212
Synonyms:
CD47, MER6, IAP, OA3
Applications:
ELISA, Flow Cyt
Recommended Dilution:
ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:
Rabbit
Isotype:
Rabbit IgG
Reactivity:
Human
Purification Method:
Purified from cell culture supernatant by affinity chromatography
Formulation:
Powder
Buffer:
1XPBS
Storage:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature.
Usage:
Research use only
Background:
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
