The Anti-CD28 Antibody (CAB20346) is a vital tool for researchers studying CD28, a co-stimulatory molecule essential for T cell activation and immune response regulation. This antibody, produced in rabbit models, exhibits high specificity towards CD28 in human samples and is validated for use in Western blot applications. By targeting the CD28 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an invaluable asset for investigations in immunology and cancer research.
CD28 is a pivotal player in immune system function, playing a crucial role in T cell activation and proliferation. Its involvement in immune response modulation makes it a key focal point for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. Understanding the mechanisms behind CD28 activation is essential for the development of innovative therapies that target or modulate immune responses in these disease contexts.
Product Name:
CD28 Rabbit Monoclonal Antibody
SKU:
CAB20346
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant protein of human CD28 Full length（P10747）.
cell surface, cytosol, external side of plasma membrane, immunological synapse, plasma membrane
Calculated MW:
25kDa
Observed MW:
45kDa
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
940
Clone Number:
ARC5082-01
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD28 antibody (CAB20346) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
