null

CD274 Rabbit Polyclonal Antibody (CAB20481)

SKU:
CAB20481
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
PD-
B7h1
Pdl1
Pdcd1l
Pdcd1l1
Pdcd1lg1
A530045L16Rik
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CD274 Rabbit Polyclonal Antibody
SKU:CAB20481
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-239 of mouse CD274 (NP_068693.1).
Sequence:FTIT APKD LYVV EYGS NVTM ECRF PVER ELDL LALV VYWE KEDE QVIQ FVAG EEDL KPQH SNFR GRAS LPKD QLLK GNAA LQIT DVKL QDAG VYCC IISY GGAD YKRI TLKV NAPY RKIN QRIS VDPA TSEH ELIC QAEG YPEA EVIW TNSD HQPV SGKR SVTT SRTE GMLL NVTS SLRV NATA NDVF YCTF WRSQ PGQN HTAE LIIP ELPA THPP QNRT H
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik; 74
Positive Sample:A-549,Mouse skeletal muscle,Rat skeletal muscle
Conjugate:Unconjugated
Cellular Localization:actin cytoskeleton, cell surface, external side of plasma membrane, extracellular exosome, nucleoplasm, plasma membrane
Calculated MW:33kDa
Observed MW:40-50kDa

The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis.

Purification Method:Affinity purification
Gene ID:60533
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from normal (control) and CD274 knockout (KO) A-549 cells, using CD274 antibody (CAB20481) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

Related Products