The Anti-CD274 Antibody (CAB20481) is a valuable tool for researchers studying CD274, a protein involved in immune regulation and co-stimulatory pathways. This polyclonal antibody is derived from rabbits and shows high reactivity with human samples, making it suitable for a variety of research applications, including Western blotting.CD274, also known as programmed death-ligand 1 (PD-L1), is a key player in immune checkpoint regulation and is known for its role in suppressing immune responses by interacting with the programmed cell death protein 1 (PD-1) receptor.
Understanding the function of CD274 is crucial for developing immunotherapies targeting this pathway, particularly in the treatment of cancer and autoimmune diseases.The Anti-CD274 Antibody enables the detection and analysis of CD274 expression in various cell types, providing researchers with valuable insights into its role in immune modulation. Its use in immunology and cancer research makes it a valuable tool for studying the mechanisms underlying immune evasion and developing targeted therapies.
Product Name:
CD274 Rabbit Polyclonal Antibody
SKU:
CAB20481
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-239 of mouse CD274 (NP_068693.1).
actin cytoskeleton, cell surface, external side of plasma membrane, extracellular exosome, nucleoplasm, plasma membrane
Calculated MW:
33kDa
Observed MW:
40-50kDa
The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis.
Purification Method:
Affinity purification
Gene ID:
60533
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and CD274 knockout (KO) A-549 cells, using CD274 antibody (CAB20481) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
