The Anti-Cathepsin B Antibody (CAB19005) is a cutting-edge tool for researchers studying Cathepsin B, a lysosomal cysteine protease involved in various cellular processes and implicated in diseases like cancer and neurodegenerative disorders. This polyclonal antibody, generated in rabbits, offers high specificity and sensitivity for detecting Cathepsin B in human samples, making it an invaluable tool for Western blot and immunohistochemistry applications.Cathepsin B is known for its role in protein degradation, antigen processing, and apoptosis, making it a key player in cellular homeostasis and disease pathology.
By targeting Cathepsin B with this antibody, researchers can gain valuable insights into its function and how it contributes to disease development. This antibody is essential for studies aiming to understand the molecular mechanisms underlying Cathepsin B activity and its potential as a therapeutic target in cancer and other diseases.
Product Name:
Cathepsin B Rabbit Monoclonal Antibody
SKU:
CAB19005
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-156 of human Cathepsin B (P07858).
This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Both Cathepsin B and Cathepsin L are involved in the cleavage of the spike protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upon its entry to the human host cell. Multiple pseudogenes of this gene have been identified.
Purification Method:
Affinity purification
Gene ID:
1508
Clone Number:
ARC0395
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Cathepsin B Rabbit mAb (CAB19005) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
