The Anti-Caspase 8 Antibody (CAB19549) is a reliable tool for studying caspase 8, a critical enzyme involved in apoptosis, or programmed cell death. This antibody, validated in knockout (KO) models, is highly specific to caspase 8 and is ideal for use in various experimental techniques such as Western blot analysis. By targeting caspase 8, researchers can gain valuable insights into the mechanisms of cell death and survival, providing a deeper understanding of diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
With its high reactivity and specificity, the Anti-Caspase 8 Antibody offers a reliable solution for investigating the role of caspase 8 in health and disease.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Purification Method:
Affinity purification
Gene ID:
841
Clone Number:
ARC0028
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Caspase-8 Rabbit mAb (CAB19549) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
