The Anti-CAD Antibody (CAB6849) is a valuable tool for researchers studying CAD, a key enzyme involved in DNA synthesis. The antibody, raised in rabbits, is highly specific to human CAD and is validated for use in various applications such as Western blot and immunohistochemistry.CAD, also known as carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase, plays a crucial role in de novo pyrimidine biosynthesis. Dysregulation of CAD expression or activity has been implicated in various diseases, including cancer and autoimmune disorders.
Therefore, the Anti-CAD Antibody is an essential tool for investigating the function and regulation of CAD in these pathological conditions.With its high specificity and versatility in different experimental techniques, the Anti-CAD Antibody is a valuable asset for researchers striving to deepen their understanding of CAD biology and its implications in human health and disease.
Product Name:
CAD Rabbit Monoclonal Antibody
SKU:
CAB6849
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CAD (P27708).
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
790
Clone Number:
ARC1426
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using CAD Rabbit mAb (CAB6849) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
