The Anti-CA125/MUC16 Antibody (CAB4666) is a valuable tool for researchers studying CA125, a cell surface glycoprotein overexpressed in ovarian cancer. This polyclonal antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications including Western blot, immunohistochemistry, and flow cytometry.CA125, also known as MUC16, is a well-known biomarker for ovarian cancer and is associated with tumor progression and poor prognosis. Detection and analysis of CA125 using this antibody can provide insights into the pathogenesis of ovarian cancer and may aid in the development of diagnostic and therapeutic strategies for this deadly disease.
The Anti-CA125/MUC16 Antibody is a valuable tool for researchers working in oncology, immunology, and biomarker discovery. Its specificity and sensitivity make it a reliable reagent for studying CA125 expression and function in cancer cells and tissues, ultimately contributing to our understanding of ovarian cancer biology and potentially leading to improved patient outcomes.
Product Name:
CA125/MUC16 Rabbit Monoclonal Antibody
SKU:
CAB4666
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 12200-12300 of human CA125 / MUC16 (NP_078966.2).
This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes.
Purification Method:
Affinity purification
Gene ID:
94025
Clone Number:
ARC1083
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from OVCAR3 cells, using CA125 / MUC16 Rabbit mAb (CAB4666) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
