The Anti-BRD4 KO Validated Antibody (CAB18839) is a powerful tool for researchers studying BRD4, a key protein involved in gene expression regulation and cell division. This antibody, generated in rabbits, shows high specificity and sensitivity for detecting BRD4 in human samples, making it suitable for Western blot applications.BRD4 is known for its role in epigenetic regulation and has been implicated in various diseases, including cancer and inflammatory disorders. By targeting BRD4, researchers can gain insights into its function and potential therapeutic strategies for these conditions.
This antibody is crucial for studies investigating the function of BRD4 in different cell types and disease models, providing valuable insights into its molecular mechanisms and potential as a target for drug development. Its validation for knockout studies ensures reliable and reproducible results, making it an essential tool for researchers in the fields of oncology, immunology, and drug discovery.
Product Name:
[KO Validated] BRD4 Rabbit Polyclonal Antibody
SKU:
CAB18839
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 650-722 of human BRD4 (NP_055114.1).
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
23476
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] BRD4 Rabbit pAb (CAB18839) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 300s.
