The Anti-Biotin Polyclonal Antibody (CAB20684) is a valuable tool for researchers studying biotin-tagged proteins in various biological samples. This antibody, raised in rabbits, exhibits high reactivity with biotin and is validated for use in applications such as immunofluorescence and flow cytometry. By specifically binding to biotin molecules, the antibody enables efficient detection and analysis of biotinylated proteins in different experimental settings.
Biotin, a vitamin essential for metabolic processes, is commonly used as a tag in protein labeling and detection experiments. The Anti-Biotin Polyclonal Antibody offers researchers a reliable means to visualize and quantify biotinylated proteins, aiding in the study of protein localization, interaction, and function. Its versatility and specificity make it a valuable asset in research areas such as cell biology, molecular biology, and biochemistry.
Product Name:
Biotin Monoclonal Antibody
SKU:
CAB20684
Size:
20uL, 100uL
Isotype:
IgG1
Host Species:
Rabbit
Reactivity:
Other (Wide Range Predicted)
Immunogen:
Biotin
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Positive Sample:
Biotin
Conjugate:
Unconjugated
Observed MW:
25-55kDa
Biotin is an organic heterobicyclic compound that consists of 2-oxohexahydro-1H-thieno[3,4-d]imidazole having a valeric acid substituent attached to the tetrahydrothiophene ring. The parent of the class of biotins. It has a role as a prosthetic group, a coenzyme, a nutraceutical, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a mouse metabolite, a cofactor and a fundamental metabolite. It is a member of biotins and a vitamin B7. It is a conjugate acid of a biotinate.
Purification Method:
Affinity purification
Clone Number:
ARC50695
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Biotin Rabbit mAb (CAB20684) at 1:300 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
